Valium Research Today is a free monthly online journal that collates and summarizes the latest research about Valium, including details on diazepam, depression, side-effects, withdrawal.

Lack of feedback inhibition on rat basolateral amygdala following stress or withdrawal from sedative-hypnotic drugs.

Isoardi NA, Bertotto ME, Martijena ID, Molina VA, Carrer HF

Departamento de Farmacología, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba and Instituto de Investigación Médica Mercedes y Martín Ferreyra, INIMEC-CONICET, Córdoba, Argentina.

Previous research has demonstrated that suppression of inhibition in projection neurons of the basolateral complex of the amygdala (BLA) represents an essential mechanism underlying the emergence of negative emotional responses, including exaggerated fear and anxiety. The present work evaluates inhibitory postsynaptic potentials (IPSPs) in pyramidal projection neurons of the BLA in rats subjected to either diazepam or ethanol withdrawal or uncontrollable stress. These are experimental paradigms conducive to a negative emotional state. In slices containing the BLA, IPSPs were studied using whole-cell patch clamp. In control animals, a small IPSP was evoked by sub-threshold stimulation of the external capsule. When an action potential (AP) was evoked by supra-threshold stimuli, IPSPs were considerably larger; these IPSPs were sensitive to blockade of GABA(A) receptors by picrotoxin. However, IPSPs were clearly reduced in diazepam- or ethanol-withdrawn and in stressed rats. Firing of an AP by a depolarizing pulse applied through the patch pipette consistently evoked an inhibitory postsynaptic current (IPSC) in the pyramidal neurons of control animals from all three experimental models; these IPSCs were mediated by GABA(A) receptor activation and were blocked after suppression of glutamatergic transmission. In contrast, no IPSCs were observed in slices from diazepam- or ethanol-withdrawn or stressed animals, although the depolarizing pulse regularly evoked an AP in pyramidal neurons. It is concluded that, in withdrawn or stressed rats, GABAergic disinhibition occurs due to attenuation or suppression of feedback inhibition.

Published 23 August 2007 in Eur J Neurosci, 26(4): 1036-44.
Full-text of this article is available online (may require subscription).

© 2004-2008 Valium Research Today. All Rights Reserved.